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We developed the web application toaSTR to help forensic experts work with MPS data simply & efficiently.

Discover MPS genotyping

Massively parallel sequencing (MPS) is emerging within the forensic community as a promising technique for high-resolution short tandem repeat (STR) genotyping, discovering both length and sequence polymorphisms. toaSTR makes bioinformatics easy and complex sequence data understandable.

User-friendly & versatile

An intuitive graphical user interface guides through the STR genotyping workflow. toaSTR handles data from various popular MPS platforms and supports both commercial and in-house multiplex PCR kits. Users can define locus-specific stutter thresholds and create custom sets of STR markers to be analyzed.

Assisted interpretation

The sequence-based stutter model predicts and identifies common stutter variants. The algorithm automatically differentiates biological (iso-)alleles from stutter and other artefacts to assist the interpretation of mixed samples.

Gain new insights

toaSTR features a comprehensive data visualization with interactive diagrams and a dynamic tabular overview of sequence observations. Results are CE-concordant and in compliance with the sequence nomenclature considerations of the International Society for Forensic Genetics (ISFG).